|
KMID : 0378020080510030016
|
|
New Medical Journal
2008 Volume.51 No. 3 p.16 ~ p.24
|
|
|
Effect of H2O2 on Glutamate Transport in Human Glioma Cells
|
|
Jung Soon-Hee
|
|
|
Abstract
|
|
|
Reactive oxygen species (ROS) have been implicated in the pathogenesis of a number of neurodegenerative disorders. However, the effect of ROS on glutamate transport in human glioma cells is not clear. This study was undertaken to examine the effect of H2O2 on glutamate uptake and to determine whether lipid peroxidation and poly (ADP-ribose) polymerase (PARP) activation are involved in the H2O2-induced transport dysfunction in A172 human glioma cells. Glutamate was transported by Na+-dependent and -independent mechanism. H2O2 inhibited the glutamate uptake in a dose-dependent manner. The inhibition was not affected by disulfide reducing agents (dithiothreitol and glutathione). The iron chelator (deferoxamine) and a hydroxyl radical scavenger (dimethylthiourea) prevented the H2O2- induced inhibition of total and Na+-independent uptake, whereas Na+-dependent uptake was not affected by these compounds. Similar results were obtained with the antioxidant Trolox. The PARP inhibitor 3-aminobenzamide prevented completely the inhibition of total and Na+-dependent uptake by H2O2. These results indicate that the H2O2-induced inhibition of
glutamate uptake is mediated by PARP activation and, in part, by lipid peroxidation in human glioma cells.
|
|
|
KEYWORD
|
|
|
Glutamate transport, ROS, Human Glioma cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|